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Cytokeratin 15 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 15 Monoclonal Antibody for WB, IF, IHC, Sandwich ELISA, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, Sandwich ELISA, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

6B4F8

Cat No : 60247-1-PBS

Print datasheet

Synonyms

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

验证数据展示

  • WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

    WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT15 antibody included
  • IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT15 antibody included
  • IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)

    IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50002-1

    Sandwich ELISA standard curve of MP50002-1

    Sandwich ELISA standard curve ofMP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: HRP-conjugated 60247-2-PBS. Standard: Ag0185. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP50002-1

    Cytometric bead array standard curve of MP50002-1

    Cytometric bead array standard curve ofMP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: 60247-2-PBS. Standard: Ag0185. Range: 1.563-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy KRT15 Ready-To-Use IHC Kit. KRT15 primary antibody included.

产品信息

60247-1-PBS targets Cytokeratin 15 as part of a matched antibody pair:

MP50002-1: 60247-1-PBS capture and 60247-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, IHC, Sandwich ELISA, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity human, mouse
Immunogen Cytokeratin 15 fusion protein Ag0185 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 15
Synonyms CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002641
Gene Symbol KRT15
Gene ID (NCBI) 3866
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19012
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.