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验证数据展示

  • WB analysis of A431 using 60247-1-Ig

    WB analysis of A431 using 60247-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 60247-1-Ig

    IHC staining of human oesophagus cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens).

    Ready-to-use IHC kits available with KRT15 antibody included
  • IHC staining of human oesophagus cancer using 60247-1-Ig

    IHC staining of human oesophagus cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens).

    Ready-to-use IHC kits available with KRT15 antibody included
  • IF Staining of HeLa using 60247-1-Ig

    IF Staining of HeLa using 60247-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

Cytokeratin 15 Monoclonal antibody

Cytokeratin 15 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60247-1-Ig

产品说明书

CloneNo. 6B4F8
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman oesophagus cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT15 Ready-To-Use IHC Kit. KRT15 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

60247-1-Ig targets Cytokeratin 15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Cytokeratin 15 fusion protein Ag0185 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 15
别名 CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC002641
基因名称 KRT15
Gene ID (NCBI) 3866
RRIDAB_2881369
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19012
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IHC protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IF protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Oxid Med Cell Longev

Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway

Authors - Jiali Li
View Article
mouseIF

Theranostics

Stimulation by exosomes from hypoxia-preconditioned hair follicle mesenchymal stem cells facilitates mitophagy by inhibiting the PI3K/AKT/mTOR signaling pathway to alleviate ulcerative colitis

Authors - Ning Li
View Article