Cytokeratin 15 Monoclonal antibody
Cytokeratin 15 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
6B4F8
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells |
Positive IHC detected in | human oesophagus cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 2 publications below |
产品信息
The immunogen of 60247-1-Ig is Cytokeratin 15 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Immunogen | Cytokeratin 15 fusion protein Ag0185 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | keratin 15 |
Synonyms | CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB |
Calculated Molecular Weight | 49 kDa |
Observed Molecular Weight | 49 kDa |
GenBank Accession Number | BC002641 |
Gene Symbol | KRT15 |
Gene ID (NCBI) | 3866 |
RRID | AB_2881369 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P19012 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 15 antibody 60247-1-Ig | Download protocol |
IHC protocol for Cytokeratin 15 antibody 60247-1-Ig | Download protocol |
IF protocol for Cytokeratin 15 antibody 60247-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway | ||
Theranostics Stimulation by exosomes from hypoxia-preconditioned hair follicle mesenchymal stem cells facilitates mitophagy by inhibiting the PI3K/AKT/mTOR signaling pathway to alleviate ulcerative colitis |