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验证数据展示

  • IF Staining of HeLa using CL488-60247

    IF Staining of HeLa using CL488-60247

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 15 antibody (CL488-60247, Clone: 6B4F8 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated Cytokeratin 15 Monoclonal antibody

Cytokeratin 15 Monoclonal Antibody for IF/ICC
Cat No. CL488-60247

产品说明书

CloneNo. 6B4F8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

KRT15, Cytokeratin-15 , Cytokeratin,Keratin, CK-15, CK15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT15 Ready-To-Use IHC Kit. KRT15 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60247-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60247 targets Cytokeratin 15 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Cytokeratin 15 fusion protein Ag0185 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 15
别名 KRT15, Cytokeratin-15 , Cytokeratin,Keratin, CK-15, CK15
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC002641
基因名称 KRT15
Gene ID (NCBI) 3866
RRIDAB_3672865
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP19012
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 15 antibody CL488-60247Download protocol
Standard Protocols
Click here to view our Standard Protocols