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IL-19 Monoclonal antibody

IL-19 Monoclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

8H10H8

Cat no : 60278-1-Ig

Print datasheet

Synonyms

IL19, interleukin19, interleukin 19, IL 19, IL 10C

验证数据展示

  • IHC staining of human tonsillitis using 60278-1-Ig

    IHC staining of human tonsillitis using 60278-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60278-1-Ig

    IHC staining of human tonsillitis using 60278-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 60278-1-Ig

    IF Staining of human tonsillitis using 60278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60278-1-Ig

    IF Staining of human tonsillitis using 60278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of U-937 using 60278-1-Ig

    IF Staining of U-937 using 60278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inU-937 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60278-1-Ig targets IL-19 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen IL-19 fusion protein Ag18887 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 19
Synonyms IL19, interleukin19, interleukin 19, IL 19, IL 10C
Calculated Molecular Weight 177 aa, 20 kDa
GenBank Accession NumberBC153127
Gene Symbol IL-19
Gene ID (NCBI) 29949
RRIDAB_2881397
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-19, a member of the IL-10 cytokine family, is expressed in epithelial cells, endothelial cells, and macrophages. It can bind the interleukin-20 receptor complex IL-20R1/IL-20R2 and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). IL-19 induces IL-6 and TNF-alpha production in monocytes. It also induces cell apoptosis and reactive oxygen species production in monocytes, which suggests a role of this cytokine in inflammatory responses. It is up-regulated in monocytes following stimulation with lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-19 alters the balance of Th1 and Th2 cells in favor of Th2 cells. IL-19 contributes to a range of diseases and disorders, such as breast cancer, squamous cell carcinoma of the skin, asthma, endotoxic shock, uremia, psoriasis, rheumatoid arthritis, and periodontal and vascular disease.

实验方案

Product Specific Protocols
WB protocol for IL-19 antibody 60278-1-IgDownload protocol
IHC protocol for IL-19 antibody 60278-1-IgDownload protocol
IF protocol for IL-19 antibody 60278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols