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验证数据展示

  • WB analysis of HeLa using 60292-1-Ig

    WB analysis of HeLa using 60292-1-Ig

    WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

  • WB analysis using 60292-1-Ig

    WB analysis using 60292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 60292-1-Ig

    IHC staining of human cervical cancer using 60292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 60292-1-Ig

    IF Staining of HeLa using 60292-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

RBM4 Monoclonal antibody

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60292-1-Ig

产品说明书

CloneNo. 3H3F12

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, fetal human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60292-1-Ig targets RBM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RBM4 fusion protein Ag2190 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 4
别名 hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC021120
基因名称 RBM4
Gene ID (NCBI) 5936
RRIDAB_2881407
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BWF3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

实验方案

Product Specific Protocols
WB protocol for RBM4 antibody 60292-1-IgDownload protocol
IHC protocol for RBM4 antibody 60292-1-IgDownload protocol
IF protocol for RBM4 antibody 60292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols