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RBM4 Monoclonal antibody

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H3F12

Cat no : 60292-1-Ig

Print datasheet

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

验证数据展示

  • WB analysis of HeLa using 60292-1-Ig

    WB analysis of HeLa using 60292-1-Ig

    WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

  • WB analysis using 60292-1-Ig

    WB analysis using 60292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 60292-1-Ig

    IHC staining of human cervical cancer using 60292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 60292-1-Ig

    IF Staining of HeLa using 60292-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, fetal human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60292-1-Ig targets RBM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen RBM4 fusion protein Ag2190 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RNA binding motif protein 4
Synonyms hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC021120
Gene Symbol RBM4
Gene ID (NCBI) 5936
RRIDAB_2881407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BWF3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

实验方案

Product Specific Protocols
WB protocol for RBM4 antibody 60292-1-IgDownload protocol
IHC protocol for RBM4 antibody 60292-1-IgDownload protocol
IF protocol for RBM4 antibody 60292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols