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  • Featured Product
  • KD/KO Validated

RBM4 Monoclonal antibody, PBS Only

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H3F12

Cat no : 60292-1-PBS

Print datasheet

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

验证数据展示

  • WB analysis of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    WB analysis of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

  • WB analysis using 60292-1-Ig (same clone as 60292-1-PBS)

    WB analysis using 60292-1-Ig (same clone as 60292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

    IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    IF Staining of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

产品信息

60292-1-PBS targets RBM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RBM4 fusion protein Ag2190 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RNA binding motif protein 4
Synonyms hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC021120
Gene Symbol RBM4
Gene ID (NCBI) 5936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BWF3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.