验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rabbit testis tissue, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60337-1-Ig targets HEXA in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, rabbit |
| 免疫原 | HEXA fusion protein Ag1854 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | hexosaminidase A (alpha polypeptide) |
| 别名 | HEXA, Hexosaminidase subunit A, TSD |
| 计算分子量 | 529 aa, 60 kDa |
| 观测分子量 | 50-54 kDa, 60-67 kDa |
| GenBank蛋白编号 | BC018927 |
| 基因名称 | HEXA |
| Gene ID (NCBI) | 3073 |
| RRID | AB_2881446 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06865 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HEXA antibody 60337-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
