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验证数据展示

  • WB analysis using 60442-2-Ig (same clone as 60442-2-PBS)

    WB analysis using 60442-2-Ig (same clone as 60442-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60442-2-Ig (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60442-2-PBS in a different storage buffer formulation.

  • WB analysis using 60442-2-Ig (same clone as 60442-2-PBS)

    WB analysis using 60442-2-Ig (same clone as 60442-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60442-2-Ig (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60442-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50598-1

    Cytometric bead array standard curve of MP50598-1

    Cytometric bead array standard curve of MP50598-1, ATAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60442-1-PBS. Detection antibody: 60442-2-PBS. Standard:Ag10471. Range: 0.098-100 ng/mL.

ATAD1 Monoclonal antibody, PBS Only (Detector)

ATAD1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 60442-2-PBS

产品说明书

CloneNo. 2A2C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, Cytometric bead array, Indirect ELISA

别名

FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60442-2-PBS targets ATAD1 as part of a matched antibody pair:

MP50598-1: 60442-1-PBS capture and 60442-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 ATAD1 fusion protein Ag10471 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase family, AAA domain containing 1
别名 FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1
计算分子量 361 aa, 41 kDa
观测分子量32-37 kDa
GenBank蛋白编号BC010868
基因名称 ATAD1
Gene ID (NCBI) 84896
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8NBU5
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.