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CD47 Monoclonal antibody

CD47 Monoclonal Antibody for IF/ICC, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4C4

Cat no : 60551-1-Ig

Print datasheet

Synonyms

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

验证数据展示

  • IF Staining of HepG2 using 60551-1-Ig

    IF Staining of HepG2 using 60551-1-Ig

    Immunofluorescent analysis of un-fixed HepG2 cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • FC experiment of human PBMCs using 60551-1-Ig

    FC experiment of human PBMCs using 60551-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 μg CD47 Monoclonal Antibody (60551-1-Ig, Clone: 1A4C4, right) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, left) was parallel stained as control. Cells were not fixed.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60551-1-Ig targets CD47 in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD47 molecule
Synonyms Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule
Calculated Molecular Weight 323 aa, 35 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ08722
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

实验方案

Product Specific Protocols
IF protocol for CD47 antibody 60551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols