Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

megathura crenulata and More (2)

Applications

WB, FC, ELISA and More (7)

Conjugate

Unconjugated

CloneNo.

1F8D3

Cat no : 66360-1-Ig

Print datasheet

Synonyms

Mouse IgG1, Kappa light chain



经过测试的应用

Positive WB detected in
Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66360-1-Ig targets Mouse IgG1 isotype control in WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA, Cell treatment applications and shows reactivity with megathura crenulata samples.

Tested Applications WB, FC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, FC, IP, CoIP, RIP, Neutralization, Cell treatment
Tested Reactivity megathura crenulata
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Mouse IgG1 isotype control
Synonyms Mouse IgG1, Kappa light chain
Gene Symbol
Gene ID (NCBI)
RRIDAB_2827991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

实验方案

Product Specific Protocols
WB protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
FC protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
CoIP

FASEB J

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection

Authors - Chujie Ding
FC

J Immunol

Phagocytic Plasma Cells in Teleost Fish Provide Insights into the Origin and Evolution of B Cells in Vertebrates

Authors - Xue-Qing Han
IP

Exp Cell Res

CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.

Authors - Yuting Yang
IHC

Am J Rhinol Allergy

Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.

Authors - Tomohiro Kawasumi

bioRxiv

Immunojanus Particles for low-volume and isolation-free unlabeled characterization of small Extracellular Vesicle in biofluids: Characterization of disease type by surface marker profiling

Authors - Sonu Kumar
RIP

Cell Biosci

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression

Authors - Liang Chen