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Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombinant Antibody

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65509-1-RR

Print datasheet

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

验证数据展示

  • FC experiment of human PBMCs using 65509-1-RR

    FC experiment of human PBMCs using 65509-1-RR

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65509-1-RR

    FC experiment of human PBMCs using 65509-1-RR

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65509-1-RR targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM-1, ICAM1, ICAM 1, BB2
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for ICAM-1/CD54 antibody 65509-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols