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CoraLite® Plus 647 Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombinant Antibody

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

15.2

Cat no : CL647-65509

Print datasheet

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

验证数据展示

  • FC experiment of human PBMCs using CL647-65509

    FC experiment of human PBMCs using CL647-65509

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD54 Rabbit Recombinant Antibody (CL647-65509, Clone:15.2) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65509

    FC experiment of human PBMCs using CL647-65509

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD54 Rabbit Recombinant Antibody (CL647-65509, Clone:15.2) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65509-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65509 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM-1, ICAM1, ICAM 1, BB2
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 ICAM-1/CD54 antibody CL647-65509Download protocol
Standard Protocols
Click here to view our Standard Protocols