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Species Reactivity
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验证数据展示

  • FC experiment of human PBMCs using 65595-1-MR

    FC experiment of human PBMCs using 65595-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD45 (HI30) (APC-65109, Clone: HI30). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 65595-1-MR

    FC experiment of human PBMCs using 65595-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody

CD18 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65595-1-MR

产品说明书

CloneNo. TS1/18

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65595-1-MR targets CD18 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human Beta 2 Integrin 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
别名 ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2
计算分子量 85 kDa
GenBank蛋白编号BC005861
基因名称 CD18
Gene ID (NCBI) 3689
ENSEMBL Gene IDENSG00000160255
RRIDAB_3670343
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

实验方案

Product Specific Protocols
FC protocol for CD18 antibody 65595-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols