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Anti-Human CD79a (HM57) Mouse IgG2a Recombinant Antibody

CD79a Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

HM57

Cat no : 65610-1-MR

Print datasheet

Synonyms

MB 1 membrane glycoprotein, MB 1, Ig-alpha, IGA, Ig alpha

验证数据展示

  • FC experiment of human PBMCs using 65610-1-MR

    FC experiment of human PBMCs using 65610-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Mouse IgG2a Recombinant Antibody (65610-1-MR, Clone: HM57) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

  • FC experiment of human PBMCs using 65610-1-MR

    FC experiment of human PBMCs using 65610-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Mouse IgG2a Recombinant Antibody (65610-1-MR, Clone: HM57) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD79a Ready-To-Use IHC Kit. CD79a primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65610-1-MR targets CD79a in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Synthetic peptide corresponding to 202-216 amino acid sequence of human CD79a 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD79a molecule, immunoglobulin-associated alpha
Synonyms MB 1 membrane glycoprotein, MB 1, Ig-alpha, IGA, Ig alpha
GenBank Accession NumberBC113731
Gene Symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
FC protocol for CD79a antibody 65610-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols