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验证数据展示

  • FC experiment of human PBMCs using 65610-1-MR

    FC experiment of human PBMCs using 65610-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a (HM57) Mouse IgG2a RecAb (65610-1-MR, Clone:HM57) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

  • FC experiment of human PBMCs using 65610-1-MR

    FC experiment of human PBMCs using 65610-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a (HM57) Mouse IgG2a RecAb (65610-1-MR, Clone:HM57)(red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4)(blue) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

Anti-Human CD79a (HM57) Mouse IgG2a Recombinant Antibody

CD79a Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 65610-1-MR

产品说明书

CloneNo. HM57

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

Membrane-bound immunoglobulin-associated protein, MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD79a Ready-To-Use IHC Kit. CD79a primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65610-1-MR targets CD79a in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Synthetic peptide corresponding to 202-216 amino acid sequence of human CD79a 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD79a molecule, immunoglobulin-associated alpha
别名 Membrane-bound immunoglobulin-associated protein, MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1
GenBank蛋白编号BC113731
基因名称 CD79A
Gene ID (NCBI) 973
RRIDAB_3670357
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
FC protocol for CD79a antibody 65610-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols