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Anti-Human CD155/PVR (SKII.4) Mouse IgG2a Recombinant Antibody

CD155/PVR Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

SKII.4

Cat no : 65632-1-MR

Print datasheet

Synonyms

PVR, Nectin like protein 5, NECL-5, NECL5, Necl 5

验证数据展示

  • FC experiment of human PBMCs using 65632-1-MR

    FC experiment of human PBMCs using 65632-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD155/PVR (SKII.4) Mouse IgG2a Recombinant Antibody (65632-1-MR, Clone: SKII.4) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65632-1-MR

    FC experiment of human PBMCs using 65632-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD155/PVR (SKII.4) Mouse IgG2a Recombinant Antibody (65632-1-MR, Clone: SKII.4) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy PVR Ready-To-Use IHC Kit. PVR primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65632-1-MR targets CD155/PVR in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen SK-N-AS human neuroblastoma cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name poliovirus receptor
Synonyms PVR, Nectin like protein 5, NECL-5, NECL5, Necl 5
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC015542
Gene Symbol CD155/PVR
Gene ID (NCBI) 5817
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

实验方案

Product Specific Protocols
FC protocol for CD155/PVR antibody 65632-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols