NOB1 Monoclonal antibody
NOB1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
8C11A12
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, human placenta tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 66048-1-Ig is NOB1 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | NOB1 fusion protein Ag17907 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) |
Synonyms | ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1 |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC000050 |
Gene Symbol | NOB1 |
Gene ID (NCBI) | 28987 |
RRID | AB_11042447 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9ULX3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NOB1 antibody 66048-1-Ig | Download protocol |
IHC protocol for NOB1 antibody 66048-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |