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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

    WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

NOB1 Monoclonal antibody, PBS Only

NOB1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66048-1-PBS

产品说明书

CloneNo. 8C11A12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66048-1-PBS targets NOB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 NOB1 fusion protein Ag17907 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
别名 ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC000050
基因名称 NOB1
Gene ID (NCBI) 28987
RRIDAB_11042447
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9ULX3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.