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NOB1 Monoclonal antibody, PBS Only

NOB1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8C11A12

Cat no : 66048-1-PBS

Print datasheet

Synonyms

ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1

验证数据展示

  • WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

    WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

产品信息

66048-1-PBS targets NOB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NOB1 fusion protein Ag17907 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
Synonyms ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC000050
Gene Symbol NOB1
Gene ID (NCBI) 28987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9ULX3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.