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验证数据展示

  • WB analysis of Jurkat using 66090-1-Ig

    WB analysis of Jurkat using 66090-1-Ig

    WB result of Stathmin antibody (66090-1-Ig, 1:4000) with si-Control and si-Stathmin transfected Jurkat cells.

  • WB analysis of Jurkat using 66090-1-Ig

    WB analysis of Jurkat using 66090-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66090-1-Ig

    IHC staining of human testis using 66090-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66090-1-Ig (Stathmin antibody at dilution of 1:50.

    Ready-to-use IHC kits available with STMN1 antibody included
  • IF Staining of mouse olfactory epithelium tissue using 66090-1-Ig

    IF Staining of mouse olfactory epithelium tissue using 66090-1-Ig

    Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of STMN1 antibody (66090-1-IG, 1:200). Basal cell staining and dendritic processes as expected. By Dr. Brian Lin (Schwob Lab).

  • Featured Product
  • KD/KO验证

Stathmin 1 Monoclonal antibody

Stathmin 1 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66090-1-Ig

产品说明书

CloneNo. 7D1E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF, IHC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, human brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STMN1 Ready-To-Use IHC Kit. STMN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66090-1-Ig targets Stathmin 1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Stathmin 1 fusion protein Ag19102 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stathmin 1/oncoprotein 18
别名
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC014353
基因名称 Stathmin 1
Gene ID (NCBI) 3925
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP16949
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.