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AFM Monoclonal antibody

AFM Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2G9F6

Cat no : 66113-1-Ig

Print datasheet

Synonyms

ALF, ALBA, ALB2, afamin/AFM, afamin

验证数据展示

  • WB analysis of human plasma using 66113-1-Ig

    WB analysis of human plasma using 66113-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66113-1-Ig

    IF Staining of HeLa using 66113-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of human plasma using 66113-1-Ig

    IP experiment of human plasma using 66113-1-Ig

    IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug.

  • IHC staining of mouse brain using 66113-1-Ig

    IHC staining of mouse brain using 66113-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66113-1-Ig

    IHC staining of mouse brain using 66113-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma
Positive IP detected inhuman plasma tissue
Positive IHC detected inmouse brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66113-1-Ig targets AFM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Immunogen AFM fusion protein Ag16866 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name afamin
Synonyms ALF, ALBA, ALB2, afamin/AFM, afamin
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2881512
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

实验方案

Product Specific Protocols
WB protocol for AFM antibody 66113-1-IgDownload protocol
IHC protocol for AFM antibody 66113-1-IgDownload protocol
IF protocol for AFM antibody 66113-1-IgDownload protocol
IP protocol for AFM antibody 66113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

J Ginseng Res

A proteomic approach reveals the differential protein expression in Drosophila melanogaster treated with red ginseng extract (Panax ginseng)

Authors - Qing-Xiu Liu
View Article