• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AFM Monoclonal antibody, PBS Only

AFM Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9F6

Cat no : 66113-1-PBS

Print datasheet

Synonyms

ALF, ALBA, ALB2, afamin/AFM, afamin

验证数据展示

  • WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

产品信息

66113-1-PBS targets AFM in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen AFM fusion protein Ag16866 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name afamin
Synonyms ALF, ALBA, ALB2, afamin/AFM, afamin
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43652
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.