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Aldolase C Monoclonal antibody

Aldolase C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5H7A5

Cat no : 66120-1-Ig

Print datasheet

Synonyms

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C

验证数据展示

  • WB analysis using 66120-1-Ig

    WB analysis using 66120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human liver cancer using 66120-1-Ig

    IHC staining of human liver cancer using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66120-1-Ig

    IHC staining of human liver cancer using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66120-1-Ig

    IF Staining of HepG2 using 66120-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of human heart using 66120-1-Ig

    WB analysis of human heart using 66120-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, HepG2 cells, K-562 cells, HSC-T6 cells, Pig brain tissue, Mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66120-1-Ig targets Aldolase C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Aldolase C fusion protein Ag6779 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldolase C, fructose-bisphosphate
Synonyms ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC003613
Gene Symbol Aldolase C
Gene ID (NCBI) 230
RRIDAB_2881519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09972
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

实验方案

Product Specific Protocols
WB protocol for Aldolase C antibody 66120-1-IgDownload protocol
IHC protocol for Aldolase C antibody 66120-1-IgDownload protocol
IF protocol for Aldolase C antibody 66120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols