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验证数据展示

  • IF Staining of HepG2 using CL488-66120

    IF Staining of HepG2 using CL488-66120

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated Aldolase C antibody (CL488-66120, Clone: 5H7A5 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated Aldolase C Monoclonal antibody

Aldolase C Monoclonal Antibody for IF/ICC
Cat No. CL488-66120

Print datasheet

CloneNo. 5H7A5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

ALDC, ALDOC, Brain type aldolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66120-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66120 targets Aldolase C in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Aldolase C fusion protein Ag6779 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldolase C, fructose-bisphosphate
Synonyms ALDC, ALDOC, Brain type aldolase
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC003613
Gene Symbol Aldolase C
Gene ID (NCBI) 230
RRIDAB_2919288
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09972
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Aldolase C antibody CL488-66120Download protocol
Standard Protocols
Click here to view our Standard Protocols