RPL3 Monoclonal antibody

RPL3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2G1B8

Cat no : 66130-1-Ig

Print datasheet

Synonyms

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B



经过测试的应用

Positive WB detected inRAW 264.7 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human lung cancer tissue, human placenta tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66130-1-Ig targets RPL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPL3 fusion protein Ag20400 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein L3
Synonyms 60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B
Calculated Molecular Weight 46/27 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012786
Gene Symbol RPL3
Gene ID (NCBI) 6122
RRIDAB_2881529
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP39023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

实验方案

Product Specific Protocols
WB protocol for RPL3 antibody 66130-1-IgDownload protocol
IHC protocol for RPL3 antibody 66130-1-IgDownload protocol
IF protocol for RPL3 antibody 66130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
humanWB

Nat Commun

Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.

Authors - Hauke S Hillen
humanWB

Elife

METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.

Authors - Eriko Matsuura-Suzuki
mouseIF

J Biol Chem

Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis.

Authors - Matthew J Brody