• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®594-conjugated RPL3 Monoclonal antibody

RPL3 Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G1B8

Cat no : CL594-66130

Print datasheet

Synonyms

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B

验证数据展示

  • WB analysis using CL594-66130

    WB analysis using CL594-66130

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66130 (RPL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using CL594-66130

    IF Staining of HepG2 using CL594-66130

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 RPL3 antibody (CL594-66130, Clone: 2G1B8 ) at dilution of 1:200.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66130-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66130 targets RPL3 in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen RPL3 fusion protein Ag20400 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein L3
Synonyms 60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B
Calculated Molecular Weight 46/27 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012786
Gene Symbol RPL3
Gene ID (NCBI) 6122
RRIDAB_2919951
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP39023
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

实验方案

Product Specific Protocols
WB protocol for CL594 RPL3 antibody CL594-66130Download protocol
IF protocol for CL594 RPL3 antibody CL594-66130Download protocol
Standard Protocols
Click here to view our Standard Protocols