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NUMBL Monoclonal antibody

NUMBL Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5F6E1

Cat no : 66155-1-Ig

Print datasheet

Synonyms

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23

验证数据展示

  • WB analysis of L02 using 66155-1-Ig

    WB analysis of L02 using 66155-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66155-1-Ig (NUMBL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66155-1-Ig

    IHC staining of mouse brain using 66155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUMBL antibody included
  • IHC staining of mouse brain using 66155-1-Ig

    IHC staining of mouse brain using 66155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUMBL antibody included
  • IF Staining of PC-12 using 66155-1-Ig

    IF Staining of PC-12 using 66155-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, L02 cells
Planning an IHC experiment? We recommend our IHCeasy NUMBL Ready-To-Use IHC Kit. NUMBL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66155-1-Ig targets NUMBL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NUMBL fusion protein Ag16771 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name numb homolog (Drosophila)-like
Synonyms CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC001794
Gene Symbol NUMBL
Gene ID (NCBI) 9253
RRIDAB_2881551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6R0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

实验方案

Product Specific Protocols
WB protocol for NUMBL antibody 66155-1-IgDownload protocol
IHC protocol for NUMBL antibody 66155-1-IgDownload protocol
IF protocol for NUMBL antibody 66155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols