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验证数据展示

  • WB analysis of L02 using 66155-1-Ig

    WB analysis of L02 using 66155-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66155-1-Ig (NUMBL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66155-1-Ig

    IHC staining of mouse brain using 66155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUMBL antibody included
  • IHC staining of mouse brain using 66155-1-Ig

    IHC staining of mouse brain using 66155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUMBL antibody included
  • IF Staining of PC-12 using 66155-1-Ig

    IF Staining of PC-12 using 66155-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

NUMBL Monoclonal antibody

NUMBL Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66155-1-Ig

产品说明书

CloneNo. 5F6E1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, L02 cells
Planning an IHC experiment? We recommend our IHCeasy NUMBL Ready-To-Use IHC Kit. NUMBL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66155-1-Ig targets NUMBL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NUMBL fusion protein Ag16771 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 numb homolog (Drosophila)-like
别名 CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23
计算分子量 65 kDa
观测分子量 70 kDa
GenBank蛋白编号BC001794
基因名称 NUMBL
Gene ID (NCBI) 9253
RRIDAB_2881551
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6R0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

实验方案

Product Specific Protocols
WB protocol for NUMBL antibody 66155-1-IgDownload protocol
IHC protocol for NUMBL antibody 66155-1-IgDownload protocol
IF protocol for NUMBL antibody 66155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols