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Host
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Conjugate
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验证数据展示

  • WB analysis of L02 using 66155-1-Ig (same clone as 66155-1-PBS)

    WB analysis of L02 using 66155-1-Ig (same clone as 66155-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66155-1-Ig (NUMBL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

    IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUMBL antibody included
  • IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

    IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUMBL antibody included
  • IF Staining of PC-12 using 66155-1-Ig (same clone as 66155-1-PBS)

    IF Staining of PC-12 using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

NUMBL Monoclonal antibody, PBS Only

NUMBL Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66155-1-PBS

产品说明书

CloneNo. 5F6E1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NUMBL Ready-To-Use IHC Kit. NUMBL primary antibody included.

产品信息

66155-1-PBS targets NUMBL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NUMBL fusion protein Ag16771 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 numb homolog (Drosophila)-like
别名 CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23
计算分子量 65 kDa
观测分子量 70 kDa
GenBank蛋白编号BC001794
基因名称 NUMBL
Gene ID (NCBI) 9253
RRIDAB_2881551
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6R0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.