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验证数据展示

  • WB analysis using 66179-1-Ig

    WB analysis using 66179-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66179-1-Ig (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human testis using 66179-1-Ig

    IHC staining of human testis using 66179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PSMD4 antibody included
  • IHC staining of human testis using 66179-1-Ig

    IHC staining of human testis using 66179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PSMD4 antibody included
  • IF Staining of HeLa using 66179-1-Ig

    IF Staining of HeLa using 66179-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD4 antibody (66179-1-Ig, Clone: 3G1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

PSMD4 Monoclonal antibody

PSMD4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66179-1-Ig

产品说明书

CloneNo. 3G1A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMD4 Ready-To-Use IHC Kit. PSMD4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66179-1-Ig targets PSMD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMD4 fusion protein Ag7010 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
别名 AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A
计算分子量 41 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002365
基因名称 PSMD4
Gene ID (NCBI) 5710
RRIDAB_2881574
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55036
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

实验方案

Product Specific Protocols
WB protocol for PSMD4 antibody 66179-1-IgDownload protocol
IHC protocol for PSMD4 antibody 66179-1-IgDownload protocol
IF protocol for PSMD4 antibody 66179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols