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验证数据展示

  • IF Staining of HeLa using CL594-66179

    IF Staining of HeLa using CL594-66179

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PSMD4 antibody (CL594-66179, Clone: 3G1A1 ) at dilution of 1:200.

CoraLite®594-conjugated PSMD4 Monoclonal antibody

PSMD4 Monoclonal Antibody for IF/ICC
Cat No. CL594-66179

产品说明书

CloneNo. 3G1A1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMD4 Ready-To-Use IHC Kit. PSMD4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66179-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66179 targets PSMD4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 PSMD4 fusion protein Ag7010 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
别名 AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A
计算分子量 41 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002365
基因名称 PSMD4
Gene ID (NCBI) 5710
RRIDAB_2883527
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP55036
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

实验方案

Product Specific Protocols
IF protocol for CL594 PSMD4 antibody CL594-66179Download protocol
Standard Protocols
Click here to view our Standard Protocols