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验证数据展示

  • IF Staining of HeLa using CL594-66179

    IF Staining of HeLa using CL594-66179

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PSMD4 antibody (CL594-66179, Clone: 3G1A1 ) at dilution of 1:200.

CoraLite®594-conjugated PSMD4 Monoclonal antibody

PSMD4 Monoclonal Antibody for IF/ICC
Cat No. CL594-66179
CloneNo. 3G1A1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMD4 Ready-To-Use IHC Kit. PSMD4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66179-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66179 targets PSMD4 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen PSMD4 fusion protein Ag7010 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Synonyms AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002365
Gene Symbol PSMD4
Gene ID (NCBI) 5710
RRIDAB_2883527
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55036
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

实验方案

Product Specific Protocols
IF protocol for CL594 PSMD4 antibody CL594-66179Download protocol
Standard Protocols
Click here to view our Standard Protocols