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PSMD4 Monoclonal antibody

PSMD4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G1A1

Cat no : 66179-1-Ig

Print datasheet

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

验证数据展示

  • WB analysis using 66179-1-Ig

    WB analysis using 66179-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66179-1-Ig (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human testis using 66179-1-Ig

    IHC staining of human testis using 66179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PSMD4 antibody included
  • IHC staining of human testis using 66179-1-Ig

    IHC staining of human testis using 66179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PSMD4 antibody included
  • IF Staining of HeLa using 66179-1-Ig

    IF Staining of HeLa using 66179-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD4 antibody (66179-1-Ig, Clone: 3G1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMD4 Ready-To-Use IHC Kit. PSMD4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66179-1-Ig targets PSMD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PSMD4 fusion protein Ag7010 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Synonyms AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002365
Gene Symbol PSMD4
Gene ID (NCBI) 5710
RRIDAB_2881574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55036
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

实验方案

Product Specific Protocols
WB protocol for PSMD4 antibody 66179-1-IgDownload protocol
IHC protocol for PSMD4 antibody 66179-1-IgDownload protocol
IF protocol for PSMD4 antibody 66179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols