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验证数据展示

  • IHC staining of human liver cancer using 66217-1-Ig

    IHC staining of human liver cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ORM2 antibody included
  • IHC staining of human liver cancer using 66217-1-Ig

    IHC staining of human liver cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ORM2 antibody included
  • WB analysis of human plasma using 66217-1-Ig

    WB analysis of human plasma using 66217-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66217-1-Ig

    IF Staining of HepG2 using 66217-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM2-Specific antibody (66217-1-Ig, Clone: 4E9H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

ORM2-Specific Monoclonal antibody

ORM2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66217-1-Ig

产品说明书

CloneNo. 4E9H10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ORM1/2, ORM2, AGPB, AGP2, AGP B'

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, A549 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ORM2 Ready-To-Use IHC Kit. ORM2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66217-1-Ig targets ORM2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ORM2-Specific fusion protein Ag18984 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 orosomucoid 2
别名 ORM1/2, ORM2, AGPB, AGP2, AGP B'
计算分子量 24 kDa
观测分子量 45 kDa
GenBank蛋白编号BC015964
基因名称 ORM2
Gene ID (NCBI) 5005
RRIDAB_2881608
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19652
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.

实验方案

Product Specific Protocols
WB protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
IHC protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
IF protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols