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验证数据展示

  • IF Staining of HepG2 using CL594-66217

    IF Staining of HepG2 using CL594-66217

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 ORM2-Specific antibody (CL594-66217, Clone: 4E9H10 ) at dilution of 1:200.

CoraLite®594-conjugated ORM2-Specific Monoclonal antibody

ORM2-Specific Monoclonal Antibody for IF/ICC
Cat No. CL594-66217

产品说明书

CloneNo. 4E9H10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

AGP 2, AGP B, AGP B', AGP2, Alpha 1 acid glycoprotein 2, OMD 2, ORM2, ORM2-Specific, orosomucoid 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ORM2 Ready-To-Use IHC Kit. ORM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66217-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66217 targets ORM2-Specific in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 ORM2-Specific fusion protein Ag18984 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 orosomucoid 2
别名 AGP 2, AGP B, AGP B', AGP2, Alpha 1 acid glycoprotein 2, OMD 2, ORM2, ORM2-Specific, orosomucoid 2
计算分子量 24 kDa
观测分子量 45 kDa
GenBank蛋白编号BC015964
基因名称 ORM2
Gene ID (NCBI) 5005
RRIDAB_3673498
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP19652
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.

实验方案

Product Specific Protocols
IF protocol for CL594 ORM2-Specific antibody CL594-66217Download protocol
Standard Protocols
Click here to view our Standard Protocols