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验证数据展示

  • IHC staining of human liver cancer using 66217-1-Ig

    IHC staining of human liver cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ORM2 antibody included
  • IHC staining of human liver cancer using 66217-1-Ig

    IHC staining of human liver cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ORM2 antibody included
  • WB analysis of human plasma using 66217-1-Ig

    WB analysis of human plasma using 66217-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66217-1-Ig

    IF Staining of HepG2 using 66217-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM2-Specific antibody (66217-1-Ig, Clone: 4E9H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

ORM2-Specific Monoclonal antibody

ORM2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66217-1-Ig

Print datasheet

CloneNo. 4E9H10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ORM1/2, ORM2, AGPB, AGP2, AGP B'

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, A549 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ORM2 Ready-To-Use IHC Kit. ORM2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66217-1-Ig targets ORM2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ORM2-Specific fusion protein Ag18984 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name orosomucoid 2
Synonyms ORM1/2, ORM2, AGPB, AGP2, AGP B'
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC015964
Gene Symbol ORM2
Gene ID (NCBI) 5005
RRIDAB_2881608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.

实验方案

Product Specific Protocols
WB protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
IHC protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
IF protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols