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  • Featured Product
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CNN2 Monoclonal antibody

CNN2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G10F5

Cat no : 66244-1-Ig

Print datasheet

Synonyms

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

验证数据展示

  • WB analysis of HeLa using 66244-1-Ig

    WB analysis of HeLa using 66244-1-Ig

    WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells.

  • WB analysis using 66244-1-Ig

    WB analysis using 66244-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human breast hyperplasia using 66244-1-Ig

    IHC staining of human breast hyperplasia using 66244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 66244-1-Ig

    IHC staining of human breast hyperplasia using 66244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, HeLa cells, pig stomach tissue, HT-1080 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66244-1-Ig targets CNN2 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, pig
Immunogen CNN2 fusion protein Ag23594 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calponin 2
Synonyms calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin
Calculated Molecular Weight 309 aa, 34 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC141818
Gene Symbol CNN2
Gene ID (NCBI) 1265
RRIDAB_2881633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

实验方案

Product Specific Protocols
WB protocol for CNN2 antibody 66244-1-IgDownload protocol
IHC protocol for CNN2 antibody 66244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols