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  • Featured Product
  • KD/KO Validated

CNN2 Monoclonal antibody, PBS Only

CNN2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10F5

Cat no : 66244-1-PBS

Print datasheet

Synonyms

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

验证数据展示

  • WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

    WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

    WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

  • WB analysis using 66244-1-Ig (same clone as 66244-1-PBS)

    WB analysis using 66244-1-Ig (same clone as 66244-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

产品信息

66244-1-PBS targets CNN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen CNN2 fusion protein Ag23594 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calponin 2
Synonyms calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin
Calculated Molecular Weight 309 aa, 34 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC141818
Gene Symbol CNN2
Gene ID (NCBI) 1265
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99439
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.