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验证数据展示

  • WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

    WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

    WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

  • WB analysis using 66244-1-Ig (same clone as 66244-1-PBS)

    WB analysis using 66244-1-Ig (same clone as 66244-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CNN2 Monoclonal antibody, PBS Only

CNN2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66244-1-PBS

产品说明书

CloneNo. 1G10F5

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, Indirect ELISA

别名

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66244-1-PBS targets CNN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 CNN2 fusion protein Ag23594 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calponin 2
别名 calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin
计算分子量 309 aa, 34 kDa
观测分子量 34-36 kDa
GenBank蛋白编号BC141818
基因名称 CNN2
Gene ID (NCBI) 1265
RRIDAB_2881633
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99439
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.