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GNAS Monoclonal antibody

GNAS Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1A9C10

Cat no : 66253-1-Ig

Print datasheet

Synonyms

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

验证数据展示

  • WB analysis of fetal human brain using 66253-1-Ig

    WB analysis of fetal human brain using 66253-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66253-1-Ig

    IHC staining of human colon using 66253-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GNAS antibody included
  • IHC staining of human colon using 66253-1-Ig

    IHC staining of human colon using 66253-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GNAS antibody included
  • IF Staining of MCF-7 using 66253-1-Ig

    IF Staining of MCF-7 using 66253-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human colon cancer using 66253-1-Ig

    IF Staining of human colon cancer using 66253-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected infetal human brain tissue, pig brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GNAS Ready-To-Use IHC Kit. GNAS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66253-1-Ig targets GNAS in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, pig
Cited Reactivityhuman, pig
Immunogen GNAS fusion protein Ag20530 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name GNAS complex locus
Synonyms Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002722
Gene Symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_2881641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95467
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

实验方案

Product Specific Protocols
WB protocol for GNAS antibody 66253-1-IgDownload protocol
IHC protocol for GNAS antibody 66253-1-IgDownload protocol
IF protocol for GNAS antibody 66253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Vet Microbiol

Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein

Authors - Jiaping Zhu
View Article
humanIF,WB

FASEB J

GRP78 acts as a cAMP/PKA signaling modulator through the MC4R pathway in porcine embryonic development

Authors - Geun Heo
View Article