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GNAS Monoclonal antibody, PBS Only

GNAS Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9C10

Cat no : 66253-1-PBS

Print datasheet

Synonyms

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

验证数据展示

  • WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

    WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GNAS antibody included
  • IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GNAS antibody included
  • IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

    IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GNAS Ready-To-Use IHC Kit. GNAS primary antibody included.

产品信息

66253-1-PBS targets GNAS in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen GNAS fusion protein Ag20530 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name GNAS complex locus
Synonyms Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002722
Gene Symbol GNAS
Gene ID (NCBI) 2778
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95467
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.