验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells |
| Positive IHC detected in | neuroblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells, Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
66254-1-Ig targets PHOX2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PHOX2B fusion protein Ag13196 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | paired-like homeobox 2b |
| 别名 | NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B |
| 计算分子量 | 31 kDa |
| 观测分子量 | 32-36 kDa |
| GenBank蛋白编号 | BC017199 |
| 基因名称 | PHOX2B |
| Gene ID (NCBI) | 8929 |
| RRID | AB_2881642 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99453 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHOX2B antibody 66254-1-Ig | Download protocol |
| IHC protocol for PHOX2B antibody 66254-1-Ig | Download protocol |
| IF protocol for PHOX2B antibody 66254-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion. | ||
Nat Biotechnol Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells |
