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PHOX2B Monoclonal antibody, PBS Only

PHOX2B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F5F5

Cat no : 66254-1-PBS

Print datasheet

Synonyms

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B

验证数据展示

  • WB analysis of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    WB analysis of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66254-1-Ig (PHOX2B Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

    IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    IF Staining of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66254-1-Ig (same clone as 66254-1-PBS)

    IF Staining of SH-SY5Y using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

产品信息

66254-1-PBS targets PHOX2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PHOX2B fusion protein Ag13196 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name paired-like homeobox 2b
Synonyms NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC017199
Gene Symbol PHOX2B
Gene ID (NCBI) 8929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99453
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.