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验证数据展示

  • WB analysis of HeLa using 66274-1-Ig

    WB analysis of HeLa using 66274-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 66274-1-Ig

    IHC staining of human skeletal muscle using 66274-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CLTCL1 antibody included
  • IHC staining of human skeletal muscle using 66274-1-Ig

    IHC staining of human skeletal muscle using 66274-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CLTCL1 antibody included
  • IF Staining of HeLa using 66274-1-Ig

    IF Staining of HeLa using 66274-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

CLTCL1 Monoclonal antibody

CLTCL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66274-1-Ig

Print datasheet

CloneNo. 1B5A10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLTCL1 Ready-To-Use IHC Kit. CLTCL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66274-1-Ig targets CLTCL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name clathrin, heavy chain-like 1
Synonyms CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD
Calculated Molecular Weight 187 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberNM_007098
Gene Symbol CLTCL1
Gene ID (NCBI) 8218
RRIDAB_2881657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53675
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.

实验方案

Product Specific Protocols
WB protocol for CLTCL1 antibody 66274-1-IgDownload protocol
IHC protocol for CLTCL1 antibody 66274-1-IgDownload protocol
IF protocol for CLTCL1 antibody 66274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols