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CoraLite® Plus 488-conjugated CLTCL1 Monoclonal antibody

CLTCL1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5A10

Cat no : CL488-66274

Print datasheet

Synonyms

CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD, FLJ36032

验证数据展示

  • IF Staining of HeLa using CL488-66274

    IF Staining of HeLa using CL488-66274

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66274 (CLTCL1 antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLTCL1 Ready-To-Use IHC Kit. CLTCL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66274-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66274 targets CLTCL1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name clathrin, heavy chain-like 1
Synonyms CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD, FLJ36032
Calculated Molecular Weight 187 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberNM_007098
Gene Symbol CLTCL1
Gene ID (NCBI) 8218
RRIDAB_2883299
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53675
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CLTCL1 antibody CL488-66274Download protocol
Standard Protocols
Click here to view our Standard Protocols