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验证数据展示

  • WB analysis of HeLa using 66277-1-Ig

    WB analysis of HeLa using 66277-1-Ig

    WB result of CAPG antibody (66277-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells.

  • WB analysis of Jurkat using 66277-1-Ig

    WB analysis of Jurkat using 66277-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66277-1-Ig

    IHC staining of human lung cancer using 66277-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 66277-1-Ig

    IHC staining of human lung cancer using 66277-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66277-1-Ig

    IF Staining of HeLa using 66277-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66277-1-Ig(CAPG antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

CAPG Monoclonal antibody

CAPG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66277-1-Ig

产品说明书

CloneNo. 3A5B2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66277-1-Ig targets CAPG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CAPG fusion protein Ag24676 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 capping protein (actin filament), gelsolin-like
别名 Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP
计算分子量 39 kDa
观测分子量 39-40 kDa
GenBank蛋白编号BC000728
基因名称 CAPG
Gene ID (NCBI) 822
RRIDAB_2881660
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP40121
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

实验方案

Product Specific Protocols
WB protocol for CAPG antibody 66277-1-IgDownload protocol
IHC protocol for CAPG antibody 66277-1-IgDownload protocol
IF protocol for CAPG antibody 66277-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols