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  • Featured Product
  • KD/KO Validated

CAPG Monoclonal antibody, PBS Only

CAPG Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A5B2

Cat no : 66277-1-PBS

Print datasheet

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

验证数据展示

  • WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    WB result of CAPG antibody (66277-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66277-1-Ig (same clone as 66277-1-PBS)

    WB analysis of Jurkat using 66277-1-Ig (same clone as 66277-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    IF Staining of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66277-1-Ig(CAPG antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

产品信息

66277-1-PBS targets CAPG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CAPG fusion protein Ag24676 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name capping protein (actin filament), gelsolin-like
Synonyms Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC000728
Gene Symbol CAPG
Gene ID (NCBI) 822
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40121
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)