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验证数据展示

  • WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    WB result of CAPG antibody (66277-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66277-1-Ig (same clone as 66277-1-PBS)

    WB analysis of Jurkat using 66277-1-Ig (same clone as 66277-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    IF Staining of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66277-1-Ig(CAPG antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CAPG Monoclonal antibody, PBS Only

CAPG Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66277-1-PBS

产品说明书

CloneNo. 3A5B2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66277-1-PBS targets CAPG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CAPG fusion protein Ag24676 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 capping protein (actin filament), gelsolin-like
别名 Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP
计算分子量 39 kDa
观测分子量 39-40 kDa
GenBank蛋白编号BC000728
基因名称 CAPG
Gene ID (NCBI) 822
RRIDAB_2881660
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP40121
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)