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  • Featured Product
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CAPG Monoclonal antibody

CAPG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A5B2

Cat no : 66277-1-Ig

Print datasheet

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

验证数据展示

  • WB analysis of HeLa using 66277-1-Ig

    WB analysis of HeLa using 66277-1-Ig

    WB result of CAPG antibody (66277-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells.

  • WB analysis of Jurkat using 66277-1-Ig

    WB analysis of Jurkat using 66277-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66277-1-Ig

    IHC staining of human lung cancer using 66277-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 66277-1-Ig

    IHC staining of human lung cancer using 66277-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66277-1-Ig

    IF Staining of HeLa using 66277-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66277-1-Ig(CAPG antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66277-1-Ig targets CAPG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CAPG fusion protein Ag24676 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name capping protein (actin filament), gelsolin-like
Synonyms Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC000728
Gene Symbol CAPG
Gene ID (NCBI) 822
RRIDAB_2881660
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

实验方案

Product Specific Protocols
WB protocol for CAPG antibody 66277-1-IgDownload protocol
IHC protocol for CAPG antibody 66277-1-IgDownload protocol
IF protocol for CAPG antibody 66277-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols