• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Caco-2 using 66311-1-Ig

    WB analysis of Caco-2 using 66311-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66311-1-Ig

    WB analysis using 66311-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HEXIM1 Monoclonal antibody

HEXIM1 Monoclonal Antibody for WB, ELISA
Cat No. 66311-1-Ig

产品说明书

CloneNo. 2C8A2

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMDA-MB-231 cells, Caco-2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy HEXIM1 Ready-To-Use IHC Kit. HEXIM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66311-1-Ig targets HEXIM1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HEXIM1 fusion protein Ag8019 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 hexamethylene bis-acetamide inducible 1
别名 Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1
计算分子量 41 kDa
观测分子量 54 kDa
GenBank蛋白编号BC006460
基因名称 HEXIM1
Gene ID (NCBI) 10614
RRIDAB_2881693
偶联类型 Unconjugated
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDO94992
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

实验方案

Product Specific Protocols
WB protocol for HEXIM1 antibody 66311-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols