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验证数据展示

  • WB analysis of Caco-2 using 66311-1-Ig (same clone as 66311-1-PBS)

    WB analysis of Caco-2 using 66311-1-Ig (same clone as 66311-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66311-1-PBS in a different storage buffer formulation.

  • WB analysis using 66311-1-Ig (same clone as 66311-1-PBS)

    WB analysis using 66311-1-Ig (same clone as 66311-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66311-1-PBS in a different storage buffer formulation.

HEXIM1 Monoclonal antibody, PBS Only

HEXIM1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66311-1-PBS
CloneNo. 2C8A2

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy HEXIM1 Ready-To-Use IHC Kit. HEXIM1 primary antibody included.

产品信息

66311-1-PBS targets HEXIM1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HEXIM1 fusion protein Ag8019 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name hexamethylene bis-acetamide inducible 1
Synonyms Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC006460
Gene Symbol HEXIM1
Gene ID (NCBI) 10614
RRIDAB_2881693
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDO94992
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).