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验证数据展示

  • WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB result of Glutamine synthetase antibody (66323-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

    IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1, Glutamine synthetase Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66323-2-PBS. Detection antibody: 66323-1-PBS. Standard:Ag6309. Range: 3.125-100 ng/mL.

  • Featured Product
  • KD/KO验证

Glutamine Synthetase Monoclonal antibody, PBS Only (Detector)

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF, Cytometric bead array, Indirect ELISA
Cat No. 66323-1-PBS

产品说明书

CloneNo. 1D10G8

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig, zebrafish

应用

WB, IHC, IF, Cytometric bead array, Indirect ELISA

别名

GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.

产品信息

66323-1-PBS targets Glutamine Synthetase as part of a matched antibody pair:

MP50483-1: 66323-2-PBS capture and 66323-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig, zebrafish
免疫原 Glutamine Synthetase fusion protein Ag6309 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate-ammonia ligase (glutamine synthetase)
别名 GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225
计算分子量 374 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC011700
基因名称 Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2881703
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15104
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).