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Glutamine Synthetase Monoclonal antibody
Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig, zebrafish and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1D10G8
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, pig liver tissue, pig brain tissue |
Positive IHC detected in | human liver cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | zebrafish retina, human brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 4 publications below |
产品信息
66323-1-Ig targets Glutamine Synthetase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, zebrafish samples.
Tested Applications | WB, IHC, IF, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, pig, zebrafish |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | Glutamine Synthetase fusion protein Ag6309 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | glutamate-ammonia ligase (glutamine synthetase) |
Synonyms | GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59 |
Calculated Molecular Weight | 374 aa, 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC011700 |
Gene Symbol | Glutamine Synthetase |
Gene ID (NCBI) | 2752 |
RRID | AB_2881703 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P15104 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Glutamine Synthetase antibody 66323-1-Ig | Download protocol |
IHC protocol for Glutamine Synthetase antibody 66323-1-Ig | Download protocol |
IF protocol for Glutamine Synthetase antibody 66323-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Acta Neuropathol Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis. | ||
In Vitro Cell Dev Biol Anim TRIM9 promotes Müller cell-derived retinal stem cells to differentiate into retinal ganglion cells by regulating Atoh7 | ||
PLoS One Excitatory to inhibitory synaptic ratios are unchanged at presymptomatic stages in multiple models of ALS |