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Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, zebrafish and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D10G8

Cat no : 66323-1-Ig

Print datasheet

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59



经过测试的应用

Positive WB detected inJurkat cells, pig liver tissue, pig brain tissue
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inzebrafish retina, human brain tissue
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66323-1-Ig targets Glutamine Synthetase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, zebrafish samples.

Tested Applications WB, IHC, IF, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, pig, zebrafish
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen Glutamine Synthetase fusion protein Ag6309 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutamate-ammonia ligase (glutamine synthetase)
Synonyms GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC011700
Gene Symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2881703
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15104
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

实验方案

Product Specific Protocols
WB protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IHC protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IF protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
mouseIF

Acta Neuropathol

Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.

Authors - Matthew J Broadhead

J Neurosci

C9ORF72 deficiency results in neurodegeneration in the zebrafish retina

Authors - Natalia Jaroszynska
zebrafishIF

Life Sci Alliance

Unique activities of two overlapping PAX6 retinal enhancers

Authors - Kirsty Uttley
ratIF

In Vitro Cell Dev Biol Anim

TRIM9 promotes Müller cell-derived retinal stem cells to differentiate into retinal ganglion cells by regulating Atoh7

Authors - Qi Zeng
mouseIF

PLoS One

Excitatory to inhibitory synaptic ratios are unchanged at presymptomatic stages in multiple models of ALS

Authors - Calum Bonthron