• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Glutamine Synthetase Monoclonal antibody, PBS Only

Glutamine Synthetase Monoclonal Antibody for WB,IF,IHC,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB,IF,IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D10G8

Cat No : 66323-1-PBS

Print datasheet

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

验证数据展示

  • WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB result of Glutamine synthetase antibody (66323-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

    IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.

产品信息

66323-1-PBS targets Glutamine Synthetase in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB,IF,IHC,ELISA Application Description
Tested Reactivity human, pig
Immunogen Glutamine Synthetase fusion protein Ag6309 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutamate-ammonia ligase (glutamine synthetase)
Synonyms GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC011700
Gene Symbol GLUL
Gene ID (NCBI) 2752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15104
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).