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  • Featured Product
  • KD/KO Validated

Glutamine Synthetase Monoclonal antibody, PBS Only (Detector)

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, zebrafish

Applications

WB, IHC, IF, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D10G8

Cat no : 66323-1-PBS

Print datasheet

Synonyms

GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225

验证数据展示

  • WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB result of Glutamine synthetase antibody (66323-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

    IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1, Glutamine synthetase Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66323-2-PBS. Detection antibody: 66323-1-PBS. Standard:Ag6309. Range: 3.125-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.

产品信息

66323-1-PBS targets Glutamine Synthetase as part of a matched antibody pair:

MP50483-1: 66323-2-PBS capture and 66323-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, pig, zebrafish
Immunogen Glutamine Synthetase fusion protein Ag6309 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutamate-ammonia ligase (glutamine synthetase)
Synonyms GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC011700
Gene Symbol Glutamine Synthetase
Gene ID (NCBI) 2752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15104
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).