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验证数据展示

  • WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66344-1-Ig (same clone as 66344-1-PBS)

    WB analysis of HeLa using 66344-1-Ig (same clone as 66344-1-PBS)

    WB result of Annexin A1 antibody (66344-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of human liver using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of human liver using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of human liver using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of human liver using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • FC experiment of K-562 using 66344-1-Ig (same clone as 66344-1-PBS)

    FC experiment of K-562 using 66344-1-Ig (same clone as 66344-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human Annexin A1 (66344-1-Ig, Clone:1E1B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66344-1-Ig (same clone as 66344-1-PBS)

    IF Staining of A549 using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (66344-1-Ig, Clone: 1E1B7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51130-1

    Cytometric bead array standard curve of MP51130-1

    Cytometric bead array standard curve of MP51130-1, ANXA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66344-2-PBS. Detection antibody: 66344-1-PBS. Standard:Ag17273. Range: 0.195-100 ng/mL.

  • Featured Product
  • KD/KO验证

Annexin A1 Monoclonal antibody, PBS Only (Detector)

Annexin A1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66344-1-PBS

产品说明书

CloneNo. 1E1B7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ANXA1 Ready-To-Use IHC Kit. ANXA1 primary antibody included.

产品信息

66344-1-PBS targets Annexin A1 as part of a matched antibody pair:

MP51130-1: 66344-2-PBS capture and 66344-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Annexin A1 fusion protein Ag17273 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 annexin A1
别名 ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26
计算分子量 346 aa, 39 kDa
观测分子量 35 kDa
GenBank蛋白编号BC001275
基因名称 Annexin A1
Gene ID (NCBI) 301
RRIDAB_2881724
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04083
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.