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验证数据展示

  • WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

    WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66378-1-Ig (same clone as 66378-1-PBS)

    WB analysis of Jurkat using 66378-1-Ig (same clone as 66378-1-PBS)

    WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

    IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

    IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51160-1

    Cytometric bead array standard curve of MP51160-1

    Cytometric bead array standard curve of MP51160-1, Occludin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66378-2-PBS. Detection antibody: 66378-1-PBS. Standard:Ag4057. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP51160-3

    Cytometric bead array standard curve of MP51160-3

    Cytometric bead array standard curve of MP51160-3, Occludin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66378-1-PBS. Detection antibody: 66378-4-PBS. Standard:Ag4057. Range: 0.098-50 ng/mL.

  • Featured Product
  • KD/KO验证

Occludin Monoclonal antibody, PBS Only (Capture/Detector)

Occludin Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66378-1-PBS

产品说明书

CloneNo. 1D3C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

OCLN, 1D3C4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66378-1-PBS targets Occludin as part of a matched antibody pair:

MP51160-1: 66378-2-PBS capture and 66378-1-PBS detection (validated in Cytometric bead array)

MP51160-3: 66378-1-PBS capture and 66378-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Occludin fusion protein Ag4057 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 occludin
别名 OCLN, 1D3C4
计算分子量 522 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC029886
基因名称 Occludin
Gene ID (NCBI) 4950
RRIDAB_2881755
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16625
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).